Guaranteed Gene Targeting and Germline Transmission Service (Full Gene Targeting Service)

Steps involved in this service

  • Gene targeting vector construction
  • Electroporation of  gene targeting vector into ES cells and apply
    drug selection
  • Pick drug-resistant ES colonies, expand ES cells and extract
    genomic DNA for gene targeting screening
  • Screening for targeted ES clones
  • Recover targeted ES clones and generate germline chimeras
  • Germline transmission confirmation
  • Delivery of mice