Gene Targeting Vector Construction
Straight knockout (KO), conditional knockout (CKO), knock-in (KI) ,  and point
mutation (PM) targeting vectors can be built on C57BL/6 or 129 genetic backgrounds.

Full Gene Targeting Service
Clients provide PTL with gene ID and anticipated modification.  PTL will generate gene targeting vector, target ES cells, generate germline chimeras, and confirm germline transmission of the modified allele in 12 months or less. Clients may choose from inbred albino C57BL/6(C2J), 129(S6)/SvEvTac, 129X1/SvJ, or hybrid 129(S6)B6 ES cell lines for their gene targeting projects.

BAC Transgenic Mice Production
BAC transgenic mice will be made by pronuclear injection of BAC transgene construct into fertilized eggs from inbred C57BL/6 or hybrid B6CBA/F1  mice. Two transgenic founders are guaranteed for each transgenic mouse production service (provided that the expression of the transgene will not cause embryonic lethality). PTL also offers service to generate BAC transgene constructs.